Nicole Natalie Trefault

Personal data

Name: Nicole Natalie Trefault Carrillo
Category: Associate Researcher
Institution Name: Universidad Mayor
Centro de Genómica y Bioinoformática
Instituto de Biotecnología
Current position : Assistant Professor
Working address:
Camino La Pirámide 5750, Huechuraba City
Santiago
Contact phones: 56-02-3281000 / extension: 5032

E-Mail address: nicole.trefault@umayor.cl
Latest Deegre or title obtained:
PhD in Molecular Genetics and Microbiology Institution where it was obtained
P. Universidad Católica de Chile

Date it was obtained: 2007
$) Signature


CV Publications since 2007
Cites source: Google Scholar and Web of Science (Gobal H Indexes 2)

Henríquez-Castillo, C., Rodríguez-Marconi, S., Rubio, F., Trefault, N., Andrade, S. and De la Iglesia, R. Eukaryotic picophytoplankton community response to copper enrichment in a metal-perturbed coastal environment. 2015. Accepted in Phycological Research.

Plominsky AM, Delherbe N, Ugalde JA, Allen EE, Blanchet M, Ikeda P, Santibañez F, Hanselmann K, Ulloa O, De la Iglesia R, von Dassow P, Astorga M, GálvezMJ, González ML, Henríquez-Castillo C, Vaulot D, Lopes do Santos A, van den Engh G, Gimpel C, Bertoglio F, Delgado Y, Docmac F, Elizondo-Patrone C, Narváez S, Sorroche F, Rojas- Herrera M, Trefault N. Metagenome sequencing of the microbial community of a solar saltern crystallizer pond at Cáhuil Lagoon, Chile. 2014. Genome Announc. 2(6):e01172- 14. doi:10.1128/genomeA.01172-14.

Kumar, A., Trefault, N., and Olaniran, S. Microbial degradation of 2, 4-dichlorophenoxyacetic acid: Insight into the enzymes and catabolic genes involved, their regulation and biotechnological implications. 2014. Critical Reviews in Microbiology, DOI: 10.3109/1040841X.2014.917068

De la Iglesia, R. and Trefault, N. Marine photosynthetic eukaryotes in polar systems: unveiling phytoplankton diversity and composition in Antarctic waters. 2012. RChHN, 85:435-443.

Boher, F., Trefault, N., Piulachs, M., Bellés, X., Godoy-Herrera, R., Bozinovic, F. (2012) Biogeographic origin and thermal acclimation interact to determine survival and hsp90 expression in Drosophila species submitted to thermal stress. Comparative Biochemistry and Physiology, Part A, doi:10.1016/j.cbpa.2012.04.017

Trefault, N., Krock, B., Delherbe, N., and Vásquez, M. (2011) Latitudinal transects in the southeastern Pacific Ocean reveal a diverse but patchy distribution of phycotoxins. Toxicon, 58:389-397. (IF: 2,451; cited 0)

Soto-Liebe, K., Murillo, A., Krock, B., Stucken, K., Fuentes-Valdés, J.J., Trefault, N., Cembella, A. and Vásquez, M. (2010) Reassessment of the toxin profile of Cylindrospermopsis raciborskii T3 and function of putative sulfotransferases on synthesis of sulfated and sulfonated PSP toxin. Toxicon 56(8):1350-61. (IF: 2,451; cited 0)

Trefault, N., Guzmán. L., Pérez, H., Godoy, M. and González, B. (2009) Involvement of different transcriptional regulators in the differencial expression of tfd genes in Cupriavidus necator JMP134. Int Microbiol 12(2):97-106. (IF: 1,8; cited 1)

Citation Indexes of N. Trefault Carrillo

Period Cites number h-index
Complete career* 85 2
2007-2012** 1 0
* Based on Google Scholar
**Cites of papers published between 2007-2012 (Based on Google Scholar)

Participation in National and International Congresses and/or Symposia

Variación temporal y espacial de bacterioplancton de vida libre y asociado a partículas en una zona costera de Chile central. Rodríguez-Marconi, S., Henríquez, C., von Dassow, P., Trefault, N., De la Iglesia, R. XXXIV Congreso Chileno de Microbiología. Valdivia, Chile. 2012.
Variación de las comunidades eucariontes pico, nano y micro fitoplancton frente a cambios de la Radiación fotosintéticamente Activa (PAR). Egas, C., Ibarra, C., De la Iglesia, R. & Trefault, N. XXXIV Congreso Chileno de Microbiología. Valdivia, Chile. 2012.
Determination of copper effect on a natural picophytoplanktonic community using a microcosm experimental approach. Henriquez-Castillo C., Rodríguez-Marconi S., Trefault N., Andrade S. & De la Iglesia R. XXI Congreso Latinoamericano de Microbiología. Santos. Brazil. 2012.
Diversity and community composition of microbial photosynthetic eukaryotes in Fildes Bay, King George Island, Antarctica. Trefault, N., De la Iglesia, R., Egas, C., Encina, G., Henríquez. C., Santibañez, J.F. and Vaulot, D. XXXII SCAR and Open Science Conference & COMNAP XXIV. Portland, USA. 2012.
Physicochemical and microbiological characterization of Fildes Bay, King George Island, Antarctica, during Summer 2012. De la Iglesia, R.; Trefault, N.; Egas, C.; Henríquez. C.; Moreno, M; Santibañez, J.F. SCAR 2012 XXXII Open Science Conference, Portland, OR, USA, July 2012.
Phytoplankton causes an increase in toxicity by production of lipophilic toxins and PSP-toxins in Chilean coastal waters. N. Trefault, N. Delherbe, B. Pérez, B. Krock, A. Cembella and M. Vásquez. 14th International Conference to Harmful Algae, 1-5 November 2010, Hersonissos, Crete-Greece.
Reassessment of the toxin profile of Cylindrospermopsis raciborskii T3 and function of putative sulfotransferases in synthesis of sulfated and sulfonated PSP Toxins. K. Soto-Liebe, K. Stucken, J.J. Fuentes-Valdés, N. Trefault, A. Cembella and M. Vásquez. 14th International Conference to Harmful Algae, 1-5 November 2010, Hersonissos, Crete-Greece.
Comparative genomic analysis of the biosynthetic pathway for saxitoxin and sulfated analogues in cyanobacteria and the dinoflagellate Alexandrium minutum. A. Cembella, K. Stucken, J. Uwe, I. Yang, B. Krock, G. Gloeckner, N. Trefault, A. Murillo, K. Soto-Liebe, J.J. Fuentes and M. Vásquez. 14th International Conference to Harmful Algae, 1-5 November 2010, Hersonissos, Crete-Greece.
Analysis of lipophilic marine toxins and PSP-toxin producing species in phytoplankton from the south eastern Pacific, Chile. Trefault, N., Krock, B., Perez, B., Delherbe, N., Mujica, J.F., Fuentealba, M. y Vásquez, M. Membership to the Society of Microbiology from Chile. XXXI Congreso Chileno de Microbiología e Inmunología. Santa Cruz, Chile. December, 2009
Comparative analysis of minimum cluster for PSP toxins biosynthesis in Raphidiopsis brookii strain D9. Murillo, A. A., Stucken, K., Soto-Liebe, K., Trefault, N., Fuentes, J. J., Cembella, A. y Vásquez M. XXXI Congreso Chileno de Microbiología e Inmunología. Santa Cruz, Chile. December, 2009
Analysis of urea and cianate metabolism in the paralytic shellfish poisoning toxin producing cyanobacterium Raphidiopsis brookii D9. Serrano, A., Trefault, N., Vásquez, M. XXXI Congreso Chileno de Microbiología e Inmunología. Santa Cruz, Chile. December, 2009
Biosynthetic pathway of the toxin cylindrospermopsin (CYN): which are the genes really part of the pathway? Soto-Liebe K., Fuentes, J. J., Stucken, K., Trefault, N., Murillo, A. y Vásquez, M. XXXI Congreso Chileno de Microbiología e Inmunología. Santa Cruz, Chile. December, 2009
Genetic and toxicological characterization of clones of the PSP-toxin producing dinoflagellate Alexandrium catenella, isolated recently from Aysen region. Trefault, N., Pérez, B., Krock, B., Delherbe, N., Mujica, J., Fuentealba, M. y Vásquez, M. XXXI Congreso Chileno de Microbiología e Inmunología. Santa Cruz, Chile. December, 2009
Analysis of genes related with urea metabolism in the paralytic shellfish poisoning toxin producing cyanobacterium Raphidiopsis sp. Serrano, A., Trefault, N., Vásquez, M. XXX Congreso Chileno de Microbiología y XI Congreso Chileno de Inmunología. Concepción, Chile. December, 2008
Metabolic cross talk for the degradation of bezoic acid derivatives in Cupriavidus necator JMP134 (pJP4). Trefault, N., Ledger, T. y González, B. 50ª Reunión Anual de la Sociedad de Biología de Chile. Pucón, November, 2007.
Expression analysis of tfd genes in Cupriavidus necator JMP134 during growth in chloroamatic pollutants. Trefault, N., Godoy, M. y González, B. XXVIII Congreso Latinoamericano de Microbiología. Pucón, October, 2006.
Several LysR transcriptional regulators control the tfd genes encoding chloroaromatic degradation in Cupriavidus necator JMP134. Trefault, N., Pérez-Bollweg, H. & González, B. XXVII Congreso Chileno de Microbiología. Pucón, October 2005.
The transcriptional regulator tfdT modulates degradation of 2,4-diclhorophenoxiacetate and 3-chlorobenzoate in Ralstonia eutropha JMP134(pJP4). Trefault, N., Herrera, J., Pérez-Bollweg, H. y González, B. XXVI Congreso Chileno de Microbiología. Valparaíso, November, 2004.
Functionality of tfd transcriptional gene regulators in Rasltonia eutropha JMP134 (pJP4). Trefault, N., Manzano, M. y González, B. XXV Congreso Chileno de Microbiología. Antofagasta, November, 2003.
Role of the catabolic plasmid pJP4 in the performance of the versatile pollutant degrading bacteria Ralstonia eutropha JMP134(pJP4): new insights from the complete sequence and annotation of pJP4. González, B., De la Iglesia, R., Ledger, T., Manzano, M., Molina, A.M., Pérez-Pantoja, D., Sánchez, M.A., Stuardo, M. y Trefault, N. Gordon Conference, 2003.
Complete sequence and annotation of the Ralstonia eutropha JMP134 catabolic plasmid pJP4. Trefault, N., De la Iglesia, R., Ledger, T., Manzano, M., Molina, A.M., Pérez- Pantoja, D., Sánchez, M.A., Stuardo, M. y González, B. XXIV Congreso Chileno de Microbiología. Punta de Tralca, November, 2002.
Integration effect of the catabolic plasmid pJP4 in Ralstonia eutropha JMP134. Trefault, N., Manzano, M., Clément, P., y González, B. XLIV Reunión Anual Sociedad de Biología de Chile. Pucón, November, 2001.
Efecto del reordenamiento de de genes tfd en el catabolismo de cloroaromáticos en Ralstonia eutropha JMP134. Clément, P., Manzano, M., Trefault, N., De la Iglesia, R., Pieper, D.H. y González, B. XXIII Reunión Anual de la Sociedad de Bioquímica y Biología Molecular de Chile. Viña del Mar, November, 2000.

Training and tutorship of students. Indicate tutor and co-tutorships, training through research seminars, regular courses among others

Tutorship of students
Mario Moreno Pino, Biotechnology undergraduate thesis, Universidad Santo Tomás Title: “Análisis de comunidades fitoplanctónicas potencialmente productoras de toxinas lipofílicas”. In progress
Claudia Egas, Biochemistry undergraduate thesis, P. Universidad Católica de Chile. “Análisis de diversidad taxonómica y funcional del comunidades fitoplanctóicas antárticas” Co-tutorshis Dr. Rodrigo De la Iglesia. In progress.
Esteban Orellana, Biology undergraduate thesis, P. Universidad Católica de Chile. “Estudio de comunidades microbianas costeras en Bahía Fildes, Antártica”. Co-tutorship Dr. Rodrigo De la Iglesia. August 2011.
Susana Rodríguez, Marine Biology undergraduate thesis. P. Universidad Católica de Chile. “Variación temporal y espacial de bacterioplancton de vida libre y asociado a partículas en una zona costera de Chile central.” Co-tutorship Dr. Rodrigo De la Iglesia. December 2011.

Training of students
Lecturer Course “Ecology and Diversity of Marine Microorganisms- ECODIM VII” (Latin American
Microbiology Postgraduate course), part of the Austral Summer Institute X, Universidad de Concepción- Estación Costera de Investigaciones Costeras.ECIM-UC, Las Cruces, Chile, Enero 9 - 28, 2012.
Lecturer and main collaborator Course Microbiology and Environmental Biotechnology (BIO-331), Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. 2011. Coordinators: Dr. Rodrigo De la Iglesia and Dra. Beatriz Díez.
Lecturer and main collaborator Course Marine Microbiology (BIO-277M), Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. 2011. Coordinator: Dr. Rodrigo De la Iglesia.
Lecturer and coordinator Course “Microbiology and Bacterial Physiology (BTE-008)”, Facultad de Recursos Naturales, Escuela de Biotecnología, Universidad Santo Tomás. 2007- 2010.
Lecturer and main collaborator Course Microbiology and Inmunology (BIO-277), Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. 2010. Coordinator: Dra. Mónica Vásquez.
Teacher assistant Course: “Ecology and Diversity of Marine Microorganisms- ECODIM VI” (Latin American Microbiology Postgraduate course), part of the Austral Summer Institute X, Universidad de Concepción. Dichato, Chile, Enero 4 - 23, 2010.
Lecturer Course Microbiology and Environmental Biotechnology (BIO-331), Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. 2009-2010.
Lecturer Course Microbiology and Inmunology (BIO-145G), Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. 2009.Lecurer Course Microbiology and Inmunology (BIO-145C), Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. 2008. Coordinator: Dra. Mónica Vásquez.
Instructor Course Microbiology and Environmental Biotechnology (BIO-331), Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. 2do semestre 2007.
Instructor Course Biomolecules and Microorganisms (BIO-151), Facultad de Ciencias Biológicas, P. Universidad Católica de Chile. 1er semestre 2007.

Participation in R&D projects indicating the source of funds, years of funding ad role within the project.

FONDECYT National Research Funding Competition 2014 Regular Competition “Naturally high pCO2 upwelling in Chile: Effects on phytoplankton communities, the coccolithophore Emiliania huxleyi, and interactions with iron and macro-nutrients.”
Co-Investigator. PI Dr. Peter von Dasow

International Cooperación Conicyt-USA 2012
“Signatures of selection in planktonic microbial communities and populations due to
changing oceans.”
Co-Investigator. PI Dr. Peter von Dasow

CNRS International Research Network 2012-France, Chile, Brasil
“Diversity, Evolution and Biotechnology of Marine Algae”
Co-Investigator.

FONDECYT National Research Funding Competition 2012 Initiation into Research
“Functional metagenomic of whole microbial communities associated to Antarctic marine invertebrates: diversity and bioactive compound synthetic capabilities.”
Principal Investigator.

FONDEF MR10I1008
“Desarrollo de un sistema para la detección y cuantificación molecular de organismos productores de toxinas paralizantes” 2012-2015. Associated Investigator. PI: Dra. Mónica Vásquez.

Chilean Antarctic Institute, INACH 2010
“Diversity and ecology of communities of phytoplanktonic eukaryotes in Antarctic coastal waters: a comparison between austral summer and winter.”
Co-Principal Investigator. PI Dr. Rodrigo De la Iglesia.

Programa CIMAR Fiordos 16, National Oceanographic Committee, CONA & Servicio Hidrográfico y Oceanográfico de la Armada, 2010
“Identification of lipophilic toxins and producing organisms in phytoplankton from inner waters between the Strait of Magellan and Cape Horn.”
Principal Investigator.

Fondef Marea Roja MR07I-1005
“Oligonucleotide Microarray development for detection and identification of PSP toxin producing microalgae”. Postdoctoral Position, Laboratory of Microbial Ecology and Environmental Toxicology, Dra. Mónica Vásquez.

Millenium Nucleus in Ecological Microbiology and Microbiology and Environmental Biotechnology, EMBA P04/P007-F. PhD student under supervision of Dr. Bernardo González.

Conicyt Doctoral Support Grant
“Regulation of tfd genes expresión in Cupriavidus necator JMP134: differential expression in distinct substrate growth” Principal investigator

Proyecto Fondecyt 1030493
“Interaction between plasmidial (pJP4) and chromosomals functions in Ralstonia eutropha JMP134 in the evolution of catabolic pathway for the bacterial degradation of chloroaromatic pollutants”
PhD student under supervision of Dr. Bernardo González

Fondecyt Líneas Complementarias 8990004
“Expression of microbial genes related with the aromatic compounds, lignin and hemicellulose catabolisms. Laboratory and environmental approaches”
Undergraduate student, under supervision of Dr. Bernardo González.


Consulting projects, studies and similar services provided to individuals, institutions and/or enterprises related to this project subjet(s) and any other data you consider relevant to the duties and role you will play in this project.

Revision of Fondef Aquaculture Division projects 2010-2012.